Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI mitigated the inflammation of IMN mice. (A) After being treated with 20 mg/kg and 100 mg/kg BAI, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) After being treated with BAI, the level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) After being treated with BAI, the serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). * p < 0.05, ** p < 0. 01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI suppressed the inflammation and oxidative stress of ZAS-induced podocytes by inactivating the AGE/RAGE pathway. (A-B) After being 10% ZAS induced for 1 h and 2 µmol/L BAI treated for 24 h, the expression of AGE (A) and RAGE (B) in MPC-5 cells were quantified. (C) After being treated with 2 µmol/L BAI and 2 µg/mL AGE-BSA for 24 h, the expression of RAGE in ZAS-induced MPC-5 cells were quantified using western blot assay. (D) The viability of ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment was determined using CCK-8 assay. (E-G) The levels of pro-inflammatory cytokines TNF-α (E), IL-6 (F), and MCP-1 (G) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. (H-I) The level of T-SOD (H) and MDA (I) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: Expressing, Western Blot, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI mitigated the inflammation and oxidative stress of IMN mice via AGE/RAGE pathway. (A) After being treated with 20 mg/kg BAI and 20 mg/kg AGE-BSA, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) The level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) The serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. (F) The LDH level in the kidney tissue of IMN mice was detected using a commercial reagent kit. (G-H) The levels of T-SOD (G) and MDA (H) in kidney tissue of IMN mice was detected by ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). ** p < 0.01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay